Figure 6: LratCre-labelled HSCs are not BM-derived. | Nature Communications

Figure 6: LratCre-labelled HSCs are not BM-derived.

From: Fate tracing reveals hepatic stellate cells as dominant contributors to liver fibrosis independent of its aetiology

Figure 6

(a–c) BM from mice expressing LratCre and mTom/mGFP Cre reporter was transplanted into lethally irradiated wild-type recipients. Untreated (n=2), 20 × CCl4 (n=1) and 3 weeks BDL (n=1) treated mice were killed 5–6 months after BM transplantation and showed no LratCre-induced mGFP expression, thus excluding a contribution of the BM to generation of LratCre-expressing HSCs. In contrast, LratCre-positive mice expressing mTom/mGFP, serving as positive control, showed abundant mGFP signal (a). Successful BMT was confirmed by the presence mTom-positive cells in the spleen (see inserts) and demonstration of F4/80-positive mGFP-expressing liver macrophages (b). HSCs isolated from BM-transplanted mice (n=1) showed no mGFP signal or by flow cytometric analysis, whereas controls showed abundant mGFP signal (n=4; c). Scale bar, 100 μm.

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