Figure 1: Only miR-1 specifically inhibits sars expression in vitro and in vivo. | Nature Communications

Figure 1: Only miR-1 specifically inhibits sars expression in vitro and in vivo.

From: miR-1 and miR-206 target different genes to have opposing roles during angiogenesis in zebrafish embryos

Figure 1

(a) Diagrams of the luc reporter constructs containing sars-3′UTRs from different species such as zebrafish (Z); mouse (M) and human (H). (b) Histograms presented the luc activity obtained from HEK-293T and C2C12 cells when miR-1 was co-transfected with plasmids as indicated. The phRL-myf5 vector served as a control group, and its luc activity was set to 1. (c) Histograms presented the luc activity obtained from C2C12 cells when miR-206 was co-transfected with plasmids as indicated. The phRL-myf5 vector served as a control group, and its luc activity was set to 1. (d) Diagrams of the luc reporter constructs containing Wt and mutated sars-3′UTRs. The mutated sequence was labelled with red. The seed sequence was blocked with green. (e) Histograms presented the luc activity obtained from embryos at 24 hpf. Plasmid phRL-myf5 vector served as a control group, and its luc activity was set to 1. The luc activity assay in panels (b), (c) and (e) are calculated from three independent experiments and presented as mean±s.d. (n=3). Student’s t-test was used to determine significant differences between each construct and control within each group (***P<0.005). (f) The sequences of pre-miR-1 and pre-miR-206 were aligned to miR-1-MO1 and miR-206-MO, respectively, as shown. WISH of miR-1 and miR-206 in WT, miR-1-MO1- and miR-206-MO-injected embryos was examined at 30 hpf. Scale bar presents 300 μm. (g) The patterns of SARS protein in WT and embryos injected with control-MO (8 ng), miR-1-MO1 (8 ng), miR-1-MO2 (8 ng), miR-206-MO (8 ng) and miR-1/206-MO (8 ng) at 48 hpf by western blot analysis. GAPDH was used as an internal control. (h) Statistical analysis of the average relative intensity of SARS protein from each group was presented. Data are calculated from three independent experiments and presented as mean±s.d. (n=3). Student’s t-test was used to determine significant differences between each construct and control within each group (***P<0.005 and **P<0.01).

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