Figure 4: CYR61-induced TEC proliferation is integrin α6-dependent. | Nature Communications

Figure 4: CYR61-induced TEC proliferation is integrin α6-dependent.

From: Thymic epithelial cell expansion through matricellular protein CYR61 boosts progenitor homing and T-cell output

Figure 4

(a) Immunoblot analysis of phospho- and total-Akt in lysates of IT76M1 stimulated with CYR61-Fc (5 μg ml−1) for the indicated times. Data is representative of three to four experiments±s.e.m. Quantification is provided on the right. *P≤0.05; **P≤0.01 (one-way ANOVA, Dunnet test) Full blots are provided in Supplementary Fig. S8. (b,c) 2DG-FTOC were cultured for 3 days in the presence of CYR61-Fc (5 μg ml−1) and Akt inhibitor MK2206 (1 μM) (b) or anti-integrin α6 or control antibodies (50 μg ml−1) (c). Cell numbers were quantified by flow cytometry (representative of two experiments±s.d.). *P≤0.05 (two-way ANOVA, Tukey HSD test).

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