Figure 6: CYR61 does not affect T-cell development per se. | Nature Communications

Figure 6: CYR61 does not affect T-cell development per se.

From: Thymic epithelial cell expansion through matricellular protein CYR61 boosts progenitor homing and T-cell output

Figure 6

(a–f) Fetal thymus (FTOC) were cultured in the presence of CYR61-Fc (5 μg ml−1) for 2 days (a,b,e) or 6 days (c,d,f). Flow cytometry analysis of CD4/CD8 distribution (a,c) and thymocyte subset number (b,d) and TEC number (e,f). Data ±s.e.m. are representative of three independent experiments. *P≤0.05; **P≤0.01 (two-tailed unpaired Student’s t-test). (g) Proliferative state of thymocytes in FTOC cultured in the presence of CYR61-Fc (5 μg ml−1) for 6 days. Data ±s.e.m. are representative of two independent experiments (h) T-cell proliferation assay. Cells were stimulated in anti-CD3-coated plates with anti-CD28 antibodies in presence of CYR61-Fc (5 μg ml−1) or Fc molecule for 72 h. Data are the mean of three experiments ±s.e.m.

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