Figure 7: CYR61 increases the hosting capacity of thymic lobes for circulating progenitors. | Nature Communications

Figure 7: CYR61 increases the hosting capacity of thymic lobes for circulating progenitors.

From: Thymic epithelial cell expansion through matricellular protein CYR61 boosts progenitor homing and T-cell output

Figure 7

(a,b) Three weeks after engraftment of CYR61 lobes and control lobes, CFSE-labelled WT-bone marrow cells were injected i.v. After 48 h, grafts were analysed for recruited Linneg CFSE+ cells (a) and for Lin+ CFSE+ cells (b). Lin:B220, CD11c, CD11b, CD19, CD3, CD4, CD8, Ly6G, PDCA1, NK1.1, EpCAM. Results from two independent experiments are pooled (n=7). *P≤0.05; **P≤0.01 (paired Student’s t-test). (c–j) FTOC were cultured in the presence of CYR61-Fc (5 μg ml−1) for 6 days. mRNA levels of CCL25 (c,d), P-selectin (e,f), IL7 (g,h) and FGF7 (i,j) were analysed by qPCR. Data±s.e.m. are representative of two independent experiments. *P≤0.05; **P≤0.01 (two-tailed unpaired Student’s t-test).

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