Figure 2: NLP-22 induces behavioural quiescence.
From: The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans
(a) Gene and protein structure of nlp-22 including overexpression construct. Animals show reduced movement (b) and feeding (c) relative to control animals overexpressing GFP (strain: TJ375), 2.5 h after nlp-22 induction. (c) Removing the signal sequence or mutating the KR or FRPG eliminates the ability of NLP-22 to reduce pumping rate (**P<0.0001, Student’s two-tailed t-test, N≥20). (d) Animals overexpressing nlp-22 cease pumping, but recover 9 h after heat shock (*P<0.001, **P<0.0001, Fisher’s exact test, N≥20 animals, two trials; Error bars represent s.e.m.).
