Figure 1: TRAF1 in neurons is upregulated with stroke.
From: TRAF1 is a critical regulator of cerebral ischaemia–reperfusion injury and neuronal death
(a) Co-staining of TRAF1 (red), NeuN (green) and nuclei (blue) demonstrates TRAF1 upregulation (arrowheads) in neurons of the ipsilateral (lower) hemisphere compared with the contralateral (upper) hemisphere 24 h after of ischaemia/reperfusion (I/R). Insets show a higher magnification view. Scale bar, 20 μm. (b) Representative images of cortices co-stained for TRAF1 (red), NeuN (green) and nuclei (DAPI, blue) after 24 or 72 h of I/R. Insets show a higher magnification view. Scale bar, 20 μm. (c) Quantification of TRAF1–NeuN co-immunostaining at the indicated time points (n=4, *P<0.05 versus the contralateral cortex; #P<0.05 versus the ipsilateral cortex after 24 h of I/R). (d) Immunoblots showing TRAF1 expression at the indicated time points following ischaemic onset (top). GAPDH served as a loading control. Bottom panel: quantification of normalized TRAF1 levels (n=6 per time point, *P<0.05 versus sham). (e) Immunoblots of TRAF1 in extracts of OGD-treated neurons (left) and quantification of TRAF1 levels (right, n=6, *P<0.05 versus control). The data represent the mean±s.d. Statistical analysis was carried out by unpaired Student’s t-test.
