Figure 7: TRAF1-mediated neuronal damage requires activation of ASK1 signalling cascades.
From: TRAF1 is a critical regulator of cerebral ischaemia–reperfusion injury and neuronal death
(a–d) TRAF1-mediated neuronal cell death is ASK1-dependent. (a,b) Cultured cortical neurons were infected with adenovirus encoding TRAF1 and dominant negative ASK1 (DN-ASK1), alone or together, and subjected to OGD for the indicated times. Cell viability (a) and LDH release (b) were assayed (n=6, *P<0.05 versus Ad-GFP, #P<0.05 versus DN-ASK1, †P<0.05 versus Ad-TRAF1). (c,d) Cultured cortical neurons were infected with adenovirus encoding short hairpin RNA targeting TRAF1 (Ad-shTRAF1) and ASK1, or both, and subjected to OGD for the indicated times. Cell viability (c) and LDH release (d) were assayed (n=6, *P<0.05 versus Ad-GFP, #P<0.05 versus Ad-shRNA, †P<0.05 versus Ad-ASK1, §P<0.05 versus Ad-shTRAF1). (e) The effect of ASK1 on the Akt pathway upon ischaemia/reperfusion. Cultured cortical neurons were infected with Ad-ASK1 or DN-ASK1, with or without subsequent exposure to OGD for 6 h. Immunoanalysis (top) and quantification (bottom) of the indicated proteins in lysates are shown (n=6, *P<P<0.05 and #P<0.05 versus Ad-GFP with and without OGD treatment, respectively). The data represent the mean±s.d. Statistical analysis was carried out by unpaired Student’s t-test.
