Figure 6: SREBP-1 is regulated by endogenous changes in miR-33 in vitro.
From: MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice
(a) RNA expression levels in Srebf2, miR-33 and Srebf1c in primary hepatocytes cultured in DMEM supplemented with 5% FBS or 5% LPDS with or without statin treatment. Values are the mean±s.e.m. (n=3 each for Srebf2 and Srebf1c, n=4–6 each for miR-33, *P<0.05 in one-way analysis of valiance test). (b) Protein levels of SREBP-1 in primary hepatocytes cultured in DMEM supplemented with 5% FBS or 5% LPDS with or without statin treatment. Representative western blot images are shown (n=4).
