Figure 3: Activation and characterization of a cryptic PTM gene cluster. | Nature Communications

Figure 3: Activation and characterization of a cryptic PTM gene cluster.

From: Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster

Figure 3

(a) The reconstructed cryptic SGR810-815 gene cluster from S. griseus. P1: gapdh(Kocuria rhizophila), P2: gapdh(S. griseus), P3: ermE*, P4: rpsL(Tsukamurella paurometabola), P5: rpsL(S. griseus), P6: rpsL(Cellulomonas flavigena). (b) Real-time PCR analysis of SGR genes in the reconstructed gene cluster and the control gene clusters. Ep, original cluster with ermE*p in front of the whole gene cluster; OC, original cluster; SG, native producer S. griseus; RC, reconstructed cluster. Error bars represent the standard errors and triplicates were performed for each reaction. (c) HPLC analysis of the extract from the S. lividans strain carrying the reconstructed gene cluster SGR810-815. *Major products, #minor products. Ultraviolet chromatographs were recorded at 260 nm. (d) HPLC analysis of single-deletion constructs. (e) HPLC analysis of three-gene and four-gene constructs (# indicates a small production of compound c).

Back to article page