Figure 2: Pericyte deficiency accelerates Aβ pathology in APPsw/0Pdgfrβ+/− mice.
From: RETRACTED ARTICLE: Pericyte loss influences Alzheimer-like neurodegeneration in mice
(a–e) Human Aβ40 and Aβ42 levels in the cortex and hippocampus (a,b) in vivo multiphoton microscopy of cortical angiograms with Texas-Red-conjugated dextran (red) and methoxy-X04-positive amyloid (green) (c,d, scale bar, 50 μm) and quantification of cerebral amyloid angiopathy (CAA) and parenchymal methoxy-X04-positive amyloid (e) in 9-month-old APPsw/0; Pdgfrβ+/+ and APPsw/0; Pdgfrβ+/− littermates. In a,b, human Aβ40 and Aβ42 levels were determined in 9-month-old Pdgfrβ+/+ and Pdgfrβ+/− mice. (f–h) Representative cortex and hippocampus sections stained with antibody against Aβ (6E10) (f,g upper panel scale bar, 400 μm; lower panel scale bar, 50 μm) and quantification of Aβ load (h) in 9-month-old APPsw/0; Pdgfrβ+/+ and APPsw/0; Pdgfrβ+/− mice. Insets in f,g: left, amyloid (green) and vascular smooth muscle cell actin (SM-actin, red) in the pial vessel on the surface of the brain; right, Aβ deposits in the hippocampus (green). In panels a,b,e,h, values are means±s.e.m., n=6 mice per group. In a,b, P<0.05 by ANOVA followed by Tukey’s post-hoc tests. NS, nonsignificant. In e,h, P<0.05 by Student’s t-test.
