Figure 3: Evidence of the functional coupling between μOR and TREK-1 in COS cells.
From: Activation of TREK-1 by morphine results in analgesia without adverse side effects
(a), I–V curves of TREK-1 channels elicited by voltage ramps (−100 to +50 mV, 800 ms) applied every 20 s on COS cells expressing the wild-type TREK-1 channel, in presence of 10 μM morphine or 10 μM arachidonic acid (AA) acutely perfused on the cell, or in control condition. (b) Characteristic current trace showing the activation of TREK-1 current, measured at 0 mV, by perfusion of 10 μM morphine followed by 10 μM arachidonic acid. (c) I–V curves acquired on COS cells expressing the mutant TREK-1.S333D channel in presence of 10 μM morphine, 10 μM AA or in control condition. (d) Mean±s.e.m. of wild-type TREK-1 and TREK-1.S333D current intensity in presence of 10 μM morphine and in control condition, recorded at 0 mV. Morphine significantly increased wild-type TREK-1 current (n=10, **P<0.005, Wilcoxon matched pairs test) but had no effect on the mutant channel (n=6, Wilcoxon matched pairs test).
