Figure 3: PIP2 induces VopR1–125 folding in solution.
From: Effectors of animal and plant pathogens use a common domain to bind host phosphoinositides
(a) 15N/1H HSQC spectra of Gβ1 (black) and His6-Gβ1-VopR1–125 (red). (b) 15N/1H HSQC spectra of Gβ1 (black) and His6-Gβ1-VopR1–125 (blue) in the presence of PI(4,5)P2-diC8 (at 1:1 molar ratio). Dashed line indicates region shown in expansion on right. All spectra were recorded at 25 °C on a 600 MHz NMR with VopR samples at 200 μM protein.
