Figure 4: VopS contains a PIP2-binding BPD.
From: Effectors of animal and plant pathogens use a common domain to bind host phosphoinositides
(a) HeLa cells 5 h after infection with effector-less Yersinia induced to express the indicated proteins. Actin (red) and DNA (Blue) are shown. Bar=20 μm. (b) Localization of eGFP-fusion protein in transfected HeLa cells. Bar=10 μm. (c) Localization of galactose-inducible eGFP-fusion proteins in BY4741 yeast cells. Bar=5 μm. (d) Localization of eGFP-fusion proteins in transfected HeLa cells. Bar=10 μm. (e) Localization of galactose-inducible VopS1–125–eGFP in MSS4 wild-type or temperature-sensitive mutant yeast at the restrictive temperature (37 °C). Bar=5 μm. (f) Localization of VopS1–125–eGFP in transfected HeLa cells 2 h after infection with Vibrio strains expressing (bottom panel), or deleted for (top panel), vpa0450. Bar=10 μm.
