Figure 5: BPDs of VPA0450, YpkA, and HopA1 bind phosphoinositides and localize to eukaryotic membranes.
From: Effectors of animal and plant pathogens use a common domain to bind host phosphoinositides
(a) The ‘core’ region of the VopR (GI:28898457) BPD (top), and the VopS (GI:28898460), VPA0450 (GI:28900305), YpkA (GI:51593847) and HopA1 (GI:28872465) aligned BPDs (bottom), with corresponding secondary structure prediction shown above: α-helices (red) and β-strands (green); and functional sequence motif indicated by asterisks. Conserved residues are highlighted yellow (mainly hydrophobic), grey (small), polar motif (blue) and functional motif (black). (b) Lipid overlay assay with purified His6-Gβ1-fusion proteins. Membranes were immunoblotted with anti-His antibodies. (c) Localization of eGFP-fusion protein in transfected HeLa cells. Bar=10 μm. (d) Localization of eGFP-fusion protein in Agrobacterium-infiltrated tomato epidermal cells. White box indicates region shown in expansion on the right. Chloroplast auto-fluorescence (red) is shown in right panel. Bar=10 μm.
