Figure 3: Continuous power and current outputs for the 13-enzyme fuel cell.
From: A high-energy-density sugar biobattery based on a synthetic enzymatic pathway
The EFC has an external load of 150 Ω and is run at room temperature. The experimental conditions are 100 mM HEPES, pH 7.5, buffer containing 15% wt/v maltodextrin, 10 mM MgCl2, 0.5 mM MnCl2, 4 mM NAD+, 0.5 mM thiamine pyrophosphate, 40 mM sodium phosphate, 5 mM DTT and non-immobilized enzyme loading conditions of 30 units of 1–4 enzymes, 10 units of 5–12 enzymes, 80 units of DI, 50 mg l−1 kanamycin, 40 mg l−1 tetracycline, 40 mg l−1 cycloheximide, 0.5 g l−1 sodium azide, 1 g l−1 BSA and 0.1% Triton X-100 in a 15-ml closed anode compartment.
