Figure 2: Phenotype of slanDCs in peripheral blood and human tissues.

(a–f) Panels are from cytospin preparations of freshly purified peripheral blood slanDCs, stained as labelled (LYS, lysozyme). Positivity is observed for all markers tested (n=5). (g–o) Sections are from human small bowel (g), reactive lymph node (h,i), normal lung (j,m), liver (k,n) and brain (l,o), and stained as labelled. M-DC8⁺ slanDCs are numerous in Peyer’s patches (g), while in reactive lymph nodes CD303⁺ pDCs (h) and CD1a⁺ iDCs (i) are negative for M-DC8. On serial sections, CD163⁺ alveolar macrophages (j,m), CD163⁺ Kupffer cells (k,n) and Iba1⁺ microglia (l,o) do not co-express M-DC8 (by DD2 staining). Sections are counterstained with Meyer’s haematoxylin. LF, lymphoid follicle. Magnification: 600X (a–f; scale bar, 33 μm), 100 × (g; scale bar, 200 μm), 400X (h,i; scale bar, 50 μm) and 200X (j–o; scale bar, 100 μm).