Figure 4: slanDCs in human TDLN and carcinoma distant metastasis.

Sections are from representative NM-TDLN (a–d; n=155) and M-TDLN (e–n,q–r; n=80), stained using the DD2 clone. slanDCs are virtually undetectable in NM-TDLN draining breast carcinoma (a), head and neck squamous cell carcinoma (b), colon carcinoma (c) and cutaneous melanoma (d). Examples of M-TDLN draining different primary sites including genital (GE; e,i), gastrointestinal (GI; f,j), urinary (UR; g,k), lung (LU; h,l), head and neck (H&N; m,q) and skin (SK; n,r), with (e–h and m,n) or without (i–l and q,r) slanDCs. (o,p,s,t) Sections are from salivary glands (SG)-draining LNs containing CKP⁺ (blue) benign epithelial inclusions and co-stained for M-DC8 (brown); DD2 reactivity is limited to HEV. (u–x) Sections are from a set of representative carcinoma metastases (n=52) from different primary sites (indicate by labels) to the liver (u), brain (v), pleura (w) and lung (x); M-DC8⁺ slanDCs are detectable only in a case with dense leukocyte infiltration (u). T, tumour cells. Sections were counterstained with Meyer’s haematoxylin. Original magnifications: 100X (a–d,u–x; scale bar, 200 μm) and 200X (e–t; scale bar, 100 μm).