Figure 5: Comparative analysis of tumour-associated DCs in M-TDLN.

(a–i) Sections are from M-TDLN and stained as indicated. In this representative case (n=5), CD303⁺ pDCs (a) and CD1a⁺ iDCs (b) are mostly expanded in their own nodal compartments, with iDCs also infiltrating the tumour (T) nests, whereas DD2⁺ slanDCs are found at the periphery of the tumour nodule (c). As shown by double staining, slanDCs are negative for CD83 (d) and CD208/DC-LAMP (e), at variance with CD1a⁺ iDCs (f), but co-express HLA-II molecule (g), CD40 (h) and TNFα (i). Sections are counterstained with Meyer’s haematoxylin (a–f and h,i) and DAPI (g). Original magnifications: 100X (a–c, scale bar, 200 μm); 200X (d,e, scale bar, 100 μm); 400X (f, scale bar, 50 μm); 600X (g–i, scale bar, 33 μm).