Figure 8: slanDCs interactions in Th2-polarized M-TDLN.
From: slanDCs selectively accumulate in carcinoma-draining lymph nodes and marginate metastatic cells
(a–d) Double staining of sections from representative M-TDLN cases (n=5) show that slanDCs are in contact with CD56+ NK cells (a, arrow head), CD66b+ neutrophils (b, arrow heads) and CD3+ T cells (c, arrow head), the latter population mainly represented by CD4+ T cells (d). Graph in (e) illustrates the quantitative analysis of cells expressing GATA3, T-Bet and Foxp3 in M-TDLN (n=10). (f with insert) slanDCs in M-TDLN were often found in close proximity to apoptotic cells/bodies as demonstrated by double staining with active caspase 3 (n=5). T, tumour cells. Sections are counterstained with Meyer’s haematoxylin. Original magnifications: 600X (a–c, scale bar, 33 μm); 400X (d, scale bar, 50 μm) and 200X (f, scale bar, 100 μm).
