Figure 2: Defects in terminal cell lumen formation in Btsz-depleted embryos. | Nature Communications

Figure 2: Defects in terminal cell lumen formation in Btsz-depleted embryos.

From: Guidance of subcellular tubulogenesis by actin under the control of a synaptotagmin-like protein and Moesin

Figure 2

Snap shots from time-lapse movies of the development of dorsal terminal cells in the embryo. (a,c) Terminal cells from control embryos expressing Dicer2 together with PLCδ-PH-GFP (a) or Ut-ABD-GFP (c). (b,d) Embryos expressing btsz-RNAi in addition to Dicer2 and the reporter constructs. The red arrowhead marks the tip of the lumen, the green arrowhead marks the leading edge of the cell. (e) Ratio of lumen-to-cell length in control, Btsz- and Talin-depleted embryos. At stage 16–17, the average tube length in control embryos was 88% (±3.5) and 88.5% (±7.7) of the length of the cell in PLCδ-PH-GFP- and Ut-ABD-GFP-expressing embryos, respectively. In Btsz-depleted cells, the average tube length was reduced to 64.4% (±16) and 64.3% (±12.4), respectively (P<0.001 in both cases by two-tailed t-test). Knockdown of Talin did not lead to a reduction in the tube-to-cell ratio (89.1%±5), (n>20 for each cross in e, error bars represent s.d.). Scale bar: (a–d) 5 μm. GFP, green fluorescent protein.

Back to article page