Figure 5: Apical membrane markers and secretion in Btsz-depleted terminal cells. | Nature Communications

Figure 5: Apical membrane markers and secretion in Btsz-depleted terminal cells.

From: Guidance of subcellular tubulogenesis by actin under the control of a synaptotagmin-like protein and Moesin

Figure 5

(a,b) Terminal cells expressing PLCδ-PH-GFP (green) were stained with antibodies against Crumbs (red). Crumbs is apically enriched in control (a) but not in btsz-RNAi (b). Localization of PLCδ-PH-GFP in control (a,c), btsz-RNAi (b,d) and moesin-RNAi (e) terminal cells. Apical localization of PLCδ-PH-GFP is disrupted in btsz-RNAi (b,d) and moesin-RNAi (e) cells. Distribution of Pio (red) in control (f) and Btsz-depleted (g) cells. Localization of β-integrin (red arrowhead in h,i) to the outer membrane in control cell (h) and in Btsz-depleted cells (i). (c–e) and (h,i) are single focal planes from confocal stacks and (a,b) and (f,g) are projections of confocal image stacks. Scale bars: (a,b,f–i) 10 μm, (c–e) 5 μm.

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