Figure 6: Localization of apical and secreted proteins in terminal cells.

(a–c) Crumbs localization in control (a) and Btsz-depleted (b,c) larval terminal cells; single focal planes. (d,e) Larval terminal cells expressing cytoplasmic GFP (green) were stained with antibodies against aPKC (red). aPKC remains associated with the luminal membrane after Btsz depletion (e). (f,g) Luminal chitin deposition in control (f) and btsz-RNAi (g) dorsal terminal cells in the embryo. Embryos expressing PLCδ-PH-GFP (green) were stained with the chitin-interacting dye CBD-564. Scale bars: (a–e) 10 μm, (f,g) 5 μm.