Figure 1: PCXSS experiment for live cells.
From: Imaging live cell in micro-liquid enclosure by X-ray laser diffraction
The XFEL beam from the SPring-8 Angstrom Compact Free-Electron Laser (SACLA) with a photon energy of 5.5 keV was focused at the sample position to a spot size of 1.5 μm × 2.0 μm using a Kirkpatrick-Baez (KB) mirror system27,28. An MLEA containing living M. lacticum cells was placed in a vacuum in the MAXIC instrument. The MLEA was scanned to shoot each enclosure with a single XFEL pulse. Because the pulse duration of the XFEL beam was as short as ~10 fs, X-ray laser diffraction occurs before the sample is destroyed3,4,5,6. Single-shot CXD patterns were recorded with a multiport charge-coupled device (MPCCD) octal sensor29.
