Figure 2: MLEA and live-dead experiment.
From: Imaging live cell in micro-liquid enclosure by X-ray laser diffraction
(a) MLEA can retain biological samples in a solution between two thin silicon nitride membranes, which separate the solution sample from a vacuum environment. In the current design, a single 10 mm × 10 mm MLEA chip contains a 10 × 10 array of enclosures. (b,c) Live-dead experiment was performed to confirm that the MLEA can preserve live cells long enough to perform a PCXSS measurement (Methods). The fluorescence microscopy images show live cells in green and dead cells in red and were taken soon after enclosure (b) and 1 h after enclosure (c). During the 1 h interval, MLEA was placed in a vacuum environment to simulate PCXSS measurement. The experiment showed that 99% of the cells are alive in MLEAs at 1 h after enclosure. Scale bars: 5 μm (in b and c). (d) A scanning electron microscopy (SEM) image of M. lacticum cells shows the typical shapes and sizes (Methods). Scale bar: 500 nm (in d).
