Figure 4: Liver and heart transduction with AAV9 and AAV2R585E mutants. | Nature Communications

Figure 4: Liver and heart transduction with AAV9 and AAV2R585E mutants.

From: Drawing a high-resolution functional map of adeno-associated virus capsid by massively parallel sequencing

Figure 4

We injected C57BL/6 wild type or Rag−/− mice with AAV-CMV-lacZ vector packaged with various AAV capsids at a dose of 3 × 1011 or 1 × 1012 vg per mouse intravenously (n=3 per group). We harvested tissues 11 days (wild type) or 6 weeks (Rag1−/−) post injection and performed X-Gal staining to determine transduction efficiency. Tissue sections were counterstained with light hematoxylin. This experiment was performed once. Scale bars, 1 mm for the heart and 200 μm for the liver.

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