Figure 3: Conversion of HSP1 into a promoter dependent on DNA bending. | Nature Communications

Figure 3: Conversion of HSP1 into a promoter dependent on DNA bending.

From: Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation

Figure 3

(a) Schematics of HSP1, LSP and two engineered promoters (EP1 and EP2) derived from HSP1. EP1 and EP2 were designed to reverse the orientation of TFAM on HSP1. (b) Representative transcription reactions with wild-type TFAM and the L6 mutant that is deficient in DNA bending. (c) Quantification of transcription reactions, with error bars representing s.d.’s from three independent experiments. Values are normalized to that of wild-type TFAM.

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