Figure 5: MT dithering in A-lattice seam-enriched MTs. | Nature Communications

Figure 5: MT dithering in A-lattice seam-enriched MTs.

From: Ectopic A-lattice seams destabilize microtubules

Figure 5

(a) A kymograph of the plus-end of a dynamic S. pombe MT grown from an A-lattice-enriched MT seed. Periods of rapid short fluctuations (<3 pixels equivalent to <384 nm) (blue bar, enlarged section of kymograph) appear as abortive elongations from the MT seed (green). (b) The % of total observation time spent in periods of such MT dithering at the plus (purple) and minus (cyan) MT ends rather than sustained growth or shrinkage was determined for the dynamic S. pombe MTs in Fig. 4 and plotted for dynamic MTs nucleated by B-lattice (red) or A-lattice-enriched seeds co-assembled with either 5 μM (light green) or 50 μM Mal3FL (dark green).

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