Table 2 Control experiments: effect of Mal3 on B-lattice single seam MT dynamics.

From: Ectopic A-lattice seams destabilize microtubules

Mal3 (nM)

Growth (mean±s.e.m. ( n ), nm s−1)

Shrinkage (mean±s.e.m. ( n ), nm s−1)

Catastrophe (frequency±CL ( n ), min−1)

Rescue (frequency±CL ( n ), min−1)

Plus end

 0

7.44±0.44 (148)

227.6±14.0 (136)

0.43±0.36/0.51 (137)

0.29±0.06/0.85 (3)

 1

7.25±0.27 (138)

240.4±14.3 (126)*

0.49±0.41/0.58 (129)

0.00±0.00/3.69 (0)

 10

6.03±0.29 (88)

316.0±26.4 (84)

0.48±0.38/0.59 (85)

0.22±0.01/1.22 (1)

 50

7.20±0.53 (97)

213.2±17.5 (88)

0.48±0.38/0.59 (82)

0.88±0.32/1.92 (6)

 100

9.12±0.38 (116)*

107.6±7.5 (108)*

0.56±0.46/0.68 (107)

4.33±3.44/5.37 (82)†

Minus end

 0

2.90±0.27 (71)

109.3±8.3 (59)

0.18±0.14/0.23 (57)

2.26±1.68/2.98 (50)

 1

3.17±0.33 (63)

113.4±9.1 (53)

0.22±0.17/0.29 (53)

2.75±1.63/4.34 (18)

 10

2.10±0.59 (17)

115.4±24.3 (14)

0.15±0.08/0.26 (14)

3.84±1.24/8.95 (5)

 50

2.94±0.78 (39)

66.2±10.3 (58)*

0.24±0.17/0.34 (33)

0.59±0.40/0.84 (30)

 100

1.53±0.25 (14)

60.6±9.5 (5)

0.03±0.01/0.06 (5)

5.52±1.79/12.89 (5)

  1. Dynamic S. pombe microtubules were grown from GMPCPP stabilized B-lattice single-seam brain tubulin microtubule seeds in the presence of low concentrations of full-length dimeric Mal 3 protein and the microtubule dynamics parameters were determined.
  2. *Growth and shrinkage rates significantly different from 0 nM Mal3 control determined by one-way ANOVA, and Tukey post test (at P< 0.05). Plus end growth (F (4, 582)=6.92, P< 0.0001) and shrinkage rates (F (4, 537)=20.14, P< 0.0001) contain significant differences. Only minus end shrinkage rates are significantly different (F (4, 184)=4.46, P=0.0018) with no differences between growth rates (F (4, 199)=1.13, P=0.34).
  3. †Rescue frequencies significantly different from 0 nM Mal3 control determined by the confidence limits assuming a Poisson distribution (P=0.05). No significant differences in catastrophe frequency were observed.
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