Table 3 Mean shrinkage rates of GDP MTs.

From: Ectopic A-lattice seams destabilize microtubules

 

ATP (μM)

Fast (plus) end (nm s−1±s.e.m. ( n))

Slow (minus) end (nm s−1±s.e.m. ( n))

A-lattice (Mal3FL)

0

10.68±0.30 (5)

2.78±0.42 (5)

B-lattice

0

2.68±0.73 (5)

0.34±0.13 (5)

B-lattice

1

55.14±5.12 (5)

7.06±2.82 (5)

  1. Mean shrinkage rates of GDP MTs held in a rigor kinesin clamp were compared before and after the release of the clamp by addition of 1 μM ATP. The shrinkage at the plus end was 29 times faster in the A-lattice enriched microtubules and significantly different from the B-lattice (t-test, P<0.0001). The slow ends of A-lattice-enriched MTs also shrank significantly faster than in the B-lattice MTs by 8 times (t-test with Welch’s correction for unequal variances, P=0.0054).
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