Table 1 Methylammonium uptake activity of Mep2 variants.

From: The TORC1 effector kinase Npr1 fine tunes the inherent activity of the Mep2 ammonium transport protein

 

Activity in triple- mep Δ cells

Activity in triple- mep Δ npr1-1 cells

 

(nmol mea per min per mg protein)

Mep2N4Q

9.3±3.1

0.8±0.4

Mep2N4Q,S457A,T459A,S460A

0.9±0.1

1.2±0.0

Mep2N4Q,S457A,T459A

0.7±0.6

0.6±0.4

Mep2N4Q,S457A,S460A

0.5±0.0

1.0±0.3

Mep2N4Q,T459A,S460A

6.3±1.3

2.0±0.6

Mep2N4Q,S457A

0.8±0.7

1.0±0.1

Mep2N4Q,S460A

11.2±0.5

0.5±0.5

Mep2N4Q,S457D

4.0±0.4

9.7±2.9

Mep2N4Q,S460D

12.6±0.0

1.9±1.7

  1. Initial rates of [14C]-methylammonium (0.5 mM) uptake were measured from proline-grown triple-mepΔ (31019b) and triple-mepΔ npr1-1 (31052c) cells transformed with YCpMep2N4Q, YCpMep2N4Q,S457A,T459A,S460A, YCpMep2N4Q,S457A,T459A, YCpMep2N4Q,S457A,S460A, YCpMep2N4Q,T459A,S460A, YCpMep2N4Q,S457A, YCpMep2N4Q,S460A, YCpMep2N4Q,S457D, or with YCpMep2N4Q,S460D. The s.d. values are indicated for the values corresponding to the averages of two to five independent experiments.
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