Table 1 Crystallographic data and statistics.
From: Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1
3D reconstruction parameters | With cAMP | Without cAMP |
|---|---|---|
Crystal plane group symmetry | p4212 | p4212 |
Crystal unit cell parameters | ||
a, b, c (Å) | 131.0, 131.0, 400.0 | 130.0, 130.0, 400.0 |
γ (°) | 90.0 | 90.0 |
Number of images | 78 | 67 |
Range of defocus (μm) | 0.655–3.077 | 0.498–2.600 |
IQ range used | 1–8 | 1–8 |
Tilt range used (°) | 0.0–46.0 | 0.0–44.6 |
In-plane resolution cutoff (Å) | 7.0 | 7.0 |
Vertical resolution cutoff (Å) | 12.0 | 12.0 |
Number of observed reflections | 42,588 | 36,314 |
Number of observed unique reflections | 24,242 | 20,574 |
Number of possible unique reflections in asymmetric unit | 4,025 | 3,911 |
Number observed unique reflections in asymmetric unit with FOM>1% | 3,409 | 3,337 |
Completeness to 45° tilt and 7.0 Å (12 Å vertical), counting reflections with FOM>1% (%) | 84.7 | 85.3 |
Number observed unique reflections in asymmetric unit with FOM>50% | 3,083 | 2,836 |
Completeness to 45° tilt and 7.0 Å (12 Å vertical), counting reflections with FOM>50% (%) | 76.6 | 72.5 |
Overall weighted phase residual (°) | 15.7 | 15.4 |
Overall weighted R-factor (%) | 33.5 | 32.3 |
