Figure 2: N terminus of EED directly interacts with PRC1 proteins.
From: The central role of EED in the orchestration of polycomb group complexes
(a) Confocal immunofluorescence analysis of EED (green) and BMI1 (red, top panels) or RING1B (red, bottom panels) in DU145 cells. DNA was counterstained with DAPI (blue). Insets are the magnified areas of co-localization. Scale bar, 10 μm. IF antibodies: mouse monoclonal anti-EED (Millipore, cat# 05–1320), anti-BMI1 and anti-RING1B antibodies. (b,c) Inhibition of biotin-RING1B and EED binding by unlabelled RING1B (b) or EZH2 (c) in the AlphaScreen assay. The EC50 values of untagged RING1B and EZH2 were 605 nM and 148 nM, respectively. Assays were repeated five times in duplicate. All error bars represent ±s.e.m. (d) Schematic domain structures and interaction analysis of EED and EED deletion mutants.
