Figure 2: FER mediates a developmentally regulated ROS maximum at the entrance to the female gametophyte. | Nature Communications

Figure 2: FER mediates a developmentally regulated ROS maximum at the entrance to the female gametophyte.

From: Reactive oxygen species mediate pollen tube rupture to release sperm for fertilization in Arabidopsis

Figure 2

(a) pFER::FER-GFP expression in floral developmental stages 12–16 (refs 40, 41). Arrows indicate filiform apparatus/synergid cell region. (b) H2DCF-DA-stained wild-type and fer-4 ovular ROS. Arrows indicate micropylar regions. (c,d) Quantitative analysis of H2DCF-DA-detected female gametophytic ROS in wild-type and fer-4 (c) and in wild-type and srn (d) ovules. (c) compares average filiform apparatus/synergid cell (FA/SC) region ROS intensity units; (d) compares % of ovules with ROS intensity above the baseline threshold set at 30 ROS intensity units. Both methods, and a third, comparing the ratio between FA/SC ROS and chalazal end ROS (around the antipodal cells), and comparisons using a second ROS-stain, HPF38,69 (Supplementary Fig. 4b,c), yielded comparable results. Quantitative data (c,d) are averages±s.e.m. of triplicate samplings (each with at least three pistils) from an analysis representative of three independent experiments; **, significant differences, P<0.01 by Student’s t-test. n=total number of ovules examined. Scale bar, 50 μm.

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