Figure 6: Ca2+-dependent pollen tube rupture in the female gametophyte.

Aniline blue-stained ovules show increasing level of pollen tube overgrowth in pollinated pistils fed with medium containing declining [Ca2+] or added EGTA (data=average±s.e.m. from triplicate samples (each with at least three pistils) representative of three independent examinations for individual treatment conditions; n=total number of ovules examined. **P≤0.01 by Student’s t-tests. Scale bar, 100 μm.