Figure 7: LRE contributes to female gametophytic ROS and is a component of the FER-RAC/ROP signaling complex.

(a) H₂DCF-DA-stained wild-type and lre-5 ovular ROS. f, funiculus, ROS occurred randomly, probably due to wound at the excision. (b) Quantification of filiform apparatus/synergid cell ROS in wild-type and lre-5 ovules. Supplementary Fig. 8 shows comparable levels of fer-4 and lre-5 (refs 14, 15) female gametophytes displayed pollen tube overgrowth. Quantitative data are averages±s.e.m. of triplicate samplings (each with at least five pistils) from an analysis representative of three independent experiments; **, significant differences, P<0.01 by Student’s t-test. n=total number of ovules examined. (c) ROP2-MBP pull down of FER-HA and HA-LRE. Immunoblot (upper) was stained by anti-HA antibodies to show the pulled down proteins. The FER pull-down result was similar to previously reported²⁵ and provides a comparison for LRE interaction with ROP2-MBP. The original scan and Ponceau-stained blot are shown in Supplementary Fig. 9.