Table 1 List of genes and enzymes in this study.
From: Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production
Genomic backgrounds | |
Name | Genotype (function of knockout) |
POlf | MatA, leucine−, uracil−, no extracellular proteases |
pex10 | POlf-Δpex10 (prevents peroxisome biogenesis) |
mfe1 | POlf-Δmfe1 (prevents β-oxidation) |
pex10 mfe1 | POlf-Δpex10 Δmfe1 (prevents peroxisome biogenesis and β-oxidation) |
Enzymatic overexpressions | |
Name | Function |
AMPD | Inhibits TCA cycle, increasing citric acid level |
ACLsubunit1 | Cleaves citric acid to acetyl-CoA |
ACLsubunit2 | Cleaves citric acid to acetyl-CoA |
MAE | Increases NADPH cofactor supply |
DGAisozyme1 | Catalyses lipid synthesis step |
DGAisozyme2 | Catalyses lipid synthesis step |
Auxotrophic markers | |
Name | Utilized for expression |
Leucine+/− | Episomally and chromosomally |
Uracil+/− | Chromosomally |
