Figure 1: Kctd10 is responsible for the heart defect of the zebrafish mutant 34c. | Nature Communications

Figure 1: Kctd10 is responsible for the heart defect of the zebrafish mutant 34c.

From: Kctd10 regulates heart morphogenesis by repressing the transcriptional activity of Tbx5a in zebrafish

Figure 1

(a,b) Bright-field micrographs of wild-type (wt) and 34c mutant embryos at 48 hpf. The mutant exhibits certain pericardial oedema. Scale bars=0.25 mm. (c–k) Fluorescence micrographs of the hearts of wild-type (c–e), 34c mutant (f–h) and embryos injected with 4 ng MO against the translation of kctd10 (i–k) at 48 hpf under the background of Tg(cmlc2:EGFP)/Tg(flk:mCherry) transgenic fish. Both the myocardium (green) and the endocardium (red) are substantially smaller in the mutant and the morphant, and no cavity is visible inside the heart lumen. Scale bar=0.1 mm. (l) A genetic map of the 34c mutant placed the affected gene between markers Z10456 (Chr5: 20.84 Mb) and rs40961672 (Chr5: 21.47 Mb) (Ensembl Zv9). (m) Sequencing result of kctd10 cDNA revealed an A-to-T transversion at position 120 of exon 2. (n,o) In situ hybridization of kctd10 in wild-type embryos. Kctd10 was expressed in the heart (arrow) at 34 and 48 hpf. (p) Kctd10 protein detected by immunofluorescence. The heart tube is indicated by arrow. (n–p) Scale bar=0.1 mm. (c–k,n–p) Ventral views with the anterior at the top.

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