Figure 5: Biological screening results of the DOS library.
From: Diversity-oriented synthesis as a tool for identifying new modulators of mitosis
(a) Representative image output from the phenotypic screen, highlighting the increase in mitotic cells for those treated with the positive control nocodazole and (S)-dosabulin. U2OS cells were stained with Hoechst (blue) and a phospho-Histone H3 (pH3) antibody (green). Data were collected on a cellomics arrayscan high-content microscope with a 20x planfluor objective; scale bar: 70 μm; (b) representative mitotic index assay data in U2OS cells for racemic dosabulin and its purified enantiomers. Data points are mean±s.e.m. of an experiment conducted in triplicate. CPD=compound; (c) Growth inhibition curves in U2OS cells assessed by sulforhodamine B assay for dosabulin and its purified enantiomers. Cells were treated with compounds for 72 h, before fixing and treating with the protein dye. Data points are mean±s.e.m. of an experiment conducted in triplicate.
