Figure 1: Predicting the role of Foxd1 and Foxo1 as mediators of the gene expression programme during the reprogramming process.

(a) Time course samples for microarray experiments (upper). MEFs were infected with retroviruses encoding the four transcription factors (Oct4, Sox2, Klf4 and c-Myc) or GFP at day 0. Hierarchical clustering analysis of 813 probe sets that had upregulated expression levels more than twofold as compared with day 0 (lower). (b) qRT–PCR analyses of Oct4, Foxd1 (Freac4) and Foxo1 in the reprogramming process. The increase in the expression levels of Foxd1 and Foxo1 preceded that of Oct4. Each mRNA level was normalized to the Gapdh level, and the value at day 0 was set to 1. Data are representative of five independent experiments.