Figure 3: TNFi-exposed Th17 cells are functionally distinct.
From: TNF-α blockade induces IL-10 expression in human CD4+ T cells
CD4+ T cells and monocytes were co-cultured with anti-CD3 mAb in the absence or presence of adalimumab for 3 days. (a) Sorted IL-17+CD4+ T cells (100,000 cells per well) from both conditions were cultured overnight and cell culture supernatants collected to test for the presence of the indicated cytokines (mean±s.e.m., n=6). (b,c) Sorted control-treated (Th17) or TNFi-exposed (TNFi-Th17) IL-17+ CD4+ T cells were added to autologous or allogeneic CD14+ monocytes at a 1:2 ratio and monocyte phenotype was assessed after 20 h by flow cytometry. Graphs show the expression of HLA-DR (MFI) (b) and the percentage of CD163+CD14+ monocytes (c) (n=6). (d) Sorted Th17 cells were added to monocytes in the absence or presence of neutralizing anti-IL-10 mAb (10 μg ml−1) and monocyte phenotype was measured; one of two experiments shown. Data in (a–c) were analysed by Wilcoxon matched-pairs signed-rank test.
