Table 1 Strains used in this study.

From: Yeast and mammalian autophagosomes exhibit distinct phosphatidylinositol 3-phosphate asymmetries

Name

Genotype

Reference/origin

SEY6210

MATα leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9

55

KVY114

SEY6210; atg9Δ::TRP1

4

KVY115

SEY6210; atg12Δ::HIS3

4

KVY117

SEY6210; atg16Δ::LEU2

4

KVY135

SEY6210; atg7Δ::HIS3

11

KVY5

SEY6210; atg8Δ::HIS3

39

SKY299

SEY6210; atg15Δ::TRP1

This study

TKY1001

SEY6210; atg18Δ::KanMX

This study

YHN31

SEY6210; atg2Δ::LEU2

This study

YNH228

SEY6210; atg3Δ::KanMX

This study

YOK161

SEY6210; vps34Δ::TRP1

11

YOK212

SEY6210; vps15Δ::TRP1

11

YT019

SEY6210; atg5Δ::LEU2

This study

YT089

SEY6210; ymr1Δ::hphNT1

This study

YT090

SEY6210; sjl3Δ::hphNT1

This study

YT092

SEY6210; ymr1Δ::kanMX6, sjl3Δ::hphNT1

This study

YT467

SEY6210 harboring p416-GFP

This study

YT468

SEY6210 harboring p416-GFP-p40phox-PX

This study

YT473

SEY6210; vps34Δ::TRP harboring p416-GFP-p40phox-PX

This study

  1. Deletion mutants were generated by a standard PCR-based method.