Figure 5: ASC-deficiency in BALB/c macrophages prevents LeTx-induced caspase-1 autoproteolysis but not pyroptosis and IL-1β secretion. | Nature Communications

Figure 5: ASC-deficiency in BALB/c macrophages prevents LeTx-induced caspase-1 autoproteolysis but not pyroptosis and IL-1β secretion.

From: Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation

Figure 5

(a–f) B6, BALB/c and BALB/c ASC−/− BMDMs were left untreated or pretreated with 5 μg ml−1 LPS for 3 h before incubation with LeTx (high, 10 μg PA+10 μg LF; low, 500 ng PA+250 ng LF) for another 3 h. Cell lysates were immunoblotted for caspase-1 and IL-1β (a,d) and culture supernatants were analysed for LDH (b,c) and secreted IL-1β (e,f). (g–i) BALB/c and BALB/c Casp1−/− BMDMs were primed with 5 μg ml−1 LPS for 3 h followed by LeTx treatment (high, 10 μg PA+10 μg LF; low, 500 ng PA+250 ng LF) for another 3 h. Levels of LDH (g) and secreted IL-1β (h) and IL-18 (i) were determined in culture supernatants. Data are representative of results from three experiments, and cytokine and LDH data are presented as mean±s.d. from a single representative experiment, with each condition performed in triplicate.

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