Figure 4: Hey1 downregulates USP11 and PML in GBM.
From: USP11 regulates PML stability to control Notch-induced malignancy in brain tumours
(a) Representative IHC staining for Hey1, USP11 and PML in consecutive slides derived from a grade II astrocytoma specimen and a grade IV GBM specimen. Scale bar, 50 μm. The boxed area is enlarged to show in the inset for visualizing PML-NBs. (b) Summary of the Hey1, USP11 and PML expression profiles in 95 grade II/III and 80 grade IV glioma patients. (c) Correlations among the Hey1, USP11 and PML expression levels in the cohort of 175 glioma patients. The P values in panels b and c were calculated by Fisher’s exact test. (d) Western blot (left panels) and reverse transcriptase (RT)–quantitative PCR (qPCR; right panels) analysis of USP11 and PML expression in U87 cells transfected with Hey1. (e) Western blot and RT–qPCR analysis of USP11 and PML expression in U251 cells transfected with Hey1. (f) Western blot and RT–qPCR analysis in U87 cells transfected with Hey1 siRNAs. (g) Western blot and RT–qPCR analysis in U251 cells transfected with Hey1 siRNAs. Data in panels d–g are mean±s.d. (**P<0.01 and ***P<0.001 by t-test) of three independent experiments.
