Figure 6: Notch acts upstream of Hey1 to induce USP11 and PML downregulation.
From: USP11 regulates PML stability to control Notch-induced malignancy in brain tumours
(a) Western blot analysis of USP11 and PML expression in U87 cells transfected with NIC (left) or treated with 10 μM DAPT for 36 h (right). (b) Western blot analysis of USP11 and PML expression in U251 cells transfected with NIC (left) or treated with 10 μM DAPT for 36 h (right). (c) Western blot analysis of USP11, PML and Notch1 expression in U87 cells transfected with indicated siRNAs. (d) Immunoprecipitation and western blot analysis of PML-I ubiquitination in U87 and U251 cells transfected with indicated constructs. (e) Immunoprecipitation and western blot analysis of PML-I ubiquitination in U87 and U251 cells transfected with indicated constructs and treated with or without DAPT. (f) Immunoprecipitation and western blot analysis of PML-I ubiquitination in U87 and U251 cells transfected with indicated constructs and siRNA. (g) Western blot analysis of USP11 and PML expression in U87 or U251 cells transfected with NIC and treated with 1 μM MG132 for 18 h. (h) Western blot analysis of USP11 and PML expression in indicated GICs transfected with NIC. (i) Western blot analysis of USP11 and PML expression in indicated GICs treated with DAPT. (j) Western blot analysis of USP11, PML and Notch1 expression in indicated GICs transfected with Notch1 siRNAs.
