Figure 7: Notch/Hey1-induced PML degradation pathway promotes GBM malignant phenotypes.

(a) Western blot analysis of PML and USP11 expression levels in U251 derivatives. These stable lines were used in experiments shown in panels b–d. (b) Proliferation assay of U251 derivatives treated with or without 10 μM DAPT. (c) Proliferation assay of U251 derivatives as indicated. (d) Soft agar colony formation assay of U251 derivatives. Representative images are shown on the left and quantitative data are on the right. Data in panels b–d are mean±s.d. (***P<0.001 by t-test of three independent experiments). (e–g) Kaplan–Meier survival curves of mice implanted with U87 cells stably expressing indicated constructs (upper panels), n=6 mice for each indicated cell line. The P values were determined by log-rank test. Representative light micrographs of brain tumours formed at indicated days after implantation are shown on the bottom panels. Brain tumours are marked by arrows. Scale bars, 2 mm. The expression levels of USP11 and PML in the U87 derivatives are shown in Supplementary Fig. 5a.