Figure 9: Generation of a USP11-resistant mutant of PML.
From: USP11 regulates PML stability to control Notch-induced malignancy in brain tumours
(a) Mapping of the USP11-interacting region on PML. The domain organization of PML is shown on the top. Interaction of USP11 with indicated PML deletion mutants in transfected 293T cells was analysed by coimmunoprecipitation assay. (b) PML 327–344 is required for interaction with USP11. 293T cells transfected with indicated constructs were analysed by coimmunoprecipitation. Of note, the amounts of PML deletion mutants used in transfection were adjusted to achieve similar expression levels of various PML proteins. (c,d) USP11, NIC and Hey1 cannot regulate the ubiquitination of PML d327-344 mutant. Immunoprecipitation and/or western blot analysis of PML-I or PML-IV ubiquitination in U87 cells transfected with indicated constructs. (e–g) Western blot analysis of PML expression levels in U87 cells transfected with indicated constructs.
