Figure 4: G4 DNA instability during C. elegans evolution. | Nature Communications

Figure 4: G4 DNA instability during C. elegans evolution.

From: A Polymerase Theta-dependent repair pathway suppresses extensive genomic instability at endogenous G4 DNA sites

Figure 4

(a) A phylogenetic tree of the C. elegans natural isolates used in this study, with (b) the observed versus expected number of G4 DNA-induced deletions in their genomes. Bristol N2 was used as a reference genome (see Methods for further details) ***P<1 × 10−5, **P<1 × 10−2, binomial test. (c) Graphic representation (analogous to Fig. 1f) of the sizes of the G4 deletions found in C. elegans natural isolates, the G4 motif is set at 0 (bp). The pie chart displays the overrepresentation of single-nucleotide homology for G4 DNA-induced simple deletions (in blue). (d) Templated insertions coinciding with G4 DNA-induced deletion formation found in C. elegans natural isolates. Matching sequences are underlined. In both cases the G4 motif was located near the right junction, further strengthening the notion that also the flank 3′ to the G-tract can serve as a template.

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