Figure 3: Comparison of NTE79QE119Q and NTE79QE84QE119Q with the wt NT dimer using 2D HSQC NMR. | Nature Communications

Figure 3: Comparison of NTE79QE119Q and NTE79QE84QE119Q with the wt NT dimer using 2D HSQC NMR.

From: Sequential pH-driven dimerization and stabilization of the N-terminal domain enables rapid spider silk formation

Figure 3

(a) Overlay of 15N-1H HSQC NMR spectra of NTE79QE119Q at pH 7.2 (green) and wt NT pH 5.5 (red). (b) Overlay of 15N-1H HSQC NMR spectra of NTE79QE84QE119Q at pH 7.2 (blue) and wt NT pH 5.5 (red). (c) Averaged backbone amide 1H and 15N chemical shift differences Δδav= between NTE79QE84QE119Q at pH 7.2, wt NT dimer at pH 5.5 and wt NT monomer at pH 7.2.

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