Figure 4: CD spectra of NT_E79QE119Q and NT_{E79QE84QE119Q}.

The residual molar ellipticity was measured at 25, 95 and 25 °C after cooling for wt NT at (a) pH 8.0 and (b) pH 5.5, NT_E79QE119Q at (c) pH 8.0 and (d) pH 5.5 and NT_{E79QE84QE119Q} at (e) pH 8.0 and (f) pH 5.5. The data are presented as smoothed averages of four measurements.